Rapid escape of new SARS-CoV-2 Omicron variants from BA.2 directed antibody responses.

Aiste Dijokaite-Guraliuc, R. Das, D. Zhou, H. Ginn, Chang Liu, Helen M. E. Duyvesteyn, J. Huo, Rungtiwa Nutalai, P. Supasa, M. Selvaraj, T. D. de Silva, M. Plowright, T. A. Newman, H. Hornsby, A. Mentzer, D. Skelly, T. Ritter, Nigel James Temperton, P. Klenerman, E. Barnes, S. Dunachie, Cornelius Roemer, T. Peacock, N. Paterson, Mark A. Williams, David Hall, E. Fry, J. Mongkolsapaya, Jingshan Ren, David I. Stuart, G. Screaton

Cell Reports, 2023

A delicate balance between antibody evasion and ACE2 affinity for Omicron BA.2.75

J. Huo, Aiste Dijokaite-Guraliuc, Chang Liu, R. Das, P. Supasa, M. Selvaraj, Rungtiwa Nutalai, D. Zhou, A. Mentzer, D. Skelly, T. Ritter, A. Amini, S. Bibi, S. Adele, Síle A. Johnson, N. Paterson, Mark A. Williams, D. Hall, M. Plowright, T. A. Newman, H. Hornsby, T. D. de Silva, Nigel James Temperton, P. Klenerman, E. Barnes, S. Dunachie, A. Pollard, T. Lambe, P. Goulder, E. Fry, J. Mongkolsapaya, Jingshan Ren, David I. Stuart, G. Screaton

Cell Reports, 2022

Humoral and cellular immune responses to Lassa fever virus in Lassa fever survivors and their exposed contacts in Southern Nigeria

Chinedu A. Ugwu, T. Olumade, E. Nwakpakpa, Venatius Onyia, E. Odeh, R. Duruiheoma, C. Ojide, Matthew Afam Eke, I. Nwafor, N. Chika-Igwenyi, Augustine M. Abu, B. Azuogu, N. Ajayi, E. Ogah, Oluwafemi Ayodeji, C. Abejegah, N. Adedosu, Nicholas E. Oyejide, S. Abah, Abiola Omidele, Winifred Ingbian, Emmanuel Osoba, P. Eromon, P. Oluniyi, Olusola A. Ogunsanya, A. Happi, P. Otuh, A. Nadesalingam, G. Carnell, N. Krause, Ernest T. Aguinam, R. Kinsley, D. Storisteanu, P. Tonks, D. Nelson, Carley E. McAlister, M. Boisen, R. Garry, E. Wright, Nigel James Temperton, S. Frost, J. Heeney, C. Happi

Scientific Reports, 2022

Influenza A (N1-N9) and Influenza B (B/Victoria and B/Yamagata) Neuraminidase Pseudotypes as Tools for Pandemic Preparedness and Improved Influenza Vaccine Design

Kelly A. S. da Costa, J. M. D. Del Rosario, Matteo Ferrari, Sneha Vishwanath, B. Asbach, R. Kinsley, Ralf Wagner, J. Heeney, G. Carnell, Nigel James Temperton

Vaccines, 2022

Neutralisation Hierarchy of SARS-CoV-2 Variants of Concern Using Standardised, Quantitative Neutralisation Assays Reveals a Correlation With Disease Severity; Towards Deciphering Protective Antibody Thresholds

D. Cantoni, M. Mayora-Neto, A. Nadesalingam, D. Wells, G. Carnell, Luis Ohlendorf, Matteo Ferrari, P. Palmer, A. Chan, Peter Smith, E. Bentley, Sebastian Einhauser, R. Wagner, M. Page, G. Raddi, H. Baxendale, J. Castillo-Olivares, J. Heeney, Nigel J. Temperton

Frontiers in Immunology, 2022

Potent cross-reactive antibodies following Omicron breakthrough in vaccinees

Rungtiwa Nutalai, D. Zhou, A. Tuekprakhon, H. Ginn, P. Supasa, Chang Liu, J. Huo, A. Mentzer, Helen M. E. Duyvesteyn, Aiste Dijokaite-Guraliuc, D. Skelly, T. Ritter, A. Amini, S. Bibi, S. Adele, Síle A. Johnson, B. Constantinides, H. Webster, Nigel James Temperton, P. Klenerman, E. Barnes, S. Dunachie, D. Crook, A. Pollard, T. Lambe, P. Goulder, N. Paterson, Mark A. Williams, David Hall, J. Mongkolsapaya, E. Fry, W. Dejnirattisai, Jingshan Ren, D. Stuart, G. Screaton, C. Conlon, A. Deeks, J. Frater, Lisa Frending, Siobhan Gardiner, Anni Jämsén, K. Jeffery, T. Malone, Eloise Phillips, Lucy Rothwell, Lizzie Stafford

Cell, 2022

C4b Binding Protein Acts as an Innate Immune Effector Against Influenza A Virus

Praveen M Varghese, Valarmathy Murugaiah, Nazar Beirag, Nigel James Temperton, H. Khan, S. Alrokayan, M. Al-Ahdal, B. Nal, F. Al-Mohanna, Robert B Sim, U. Kishore

Frontiers in Immunology, 2021

Correlation of Influenza B Haemagglutination Inhibiton, Single-Radial Haemolysis and Pseudotype-Based Microneutralisation Assays for Immunogenicity Testing of Seasonal Vaccines

G. Carnell, C. Trombetta, F. Ferrara, E. Montomoli, N. Temperton

Vaccines, 2021

Development of Lentiviral Vectors Pseudotyped With Influenza B Hemagglutinins: Application in Vaccine Immunogenicity, mAb Potency, and Sero-Surveillance Studies

F. Ferrara, J. M. D. Del Rosario, Kelly A S da Costa, R. Kinsley, S. Scott, S. Fereidouni, C. Thompson, P. Kellam, S. Gilbert, G. Carnell, N. Temperton

Frontiers in Immunology, 2021

Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection

J. M. D. Del Rosario, Kelly A S da Costa, B. Asbach, F. Ferrara, Matteo Ferrari, D. Wells, G. S. Mann, V. O. Ameh, C. Sabeta, A. Banyard, R. Kinsley, S. Scott, R. Wagner, J. Heeney, G. Carnell, N. Temperton

Vaccines, 2021

Nanobodies mapped to cross-reactive and divergent epitopes on A(H7N9) influenza hemagglutinin using yeast display

T. Gaiotto, W. Ramage, C. Ball, P. Risley, G. Carnell, Nigel James Temperton, O. Engelhardt, S. Hufton

Scientific Reports, 2021

Production, Titration, Neutralisation, Storage and Lyophilisation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Lentiviral Pseudotypes.

C. Genova, A. Sampson, S. Scott, D. Cantoni, M. Neto, E. Bentley, G. Mattiuzzo, E. Wright, Mariliza Derveni, B. Auld, Billy Ferrara, Dale Harrison, Mohamed Said, Arwa Z. Selim, Elinor P. Thompson, C. Thompson, G. Carnell, Nigel J. Temperton

Bio-protocol, 2021

Pseudotyped Bat Coronavirus RaTG13 is efficiently neutralised by convalescent sera from SARS-CoV-2 infected patients

D. Cantoni, M. Mayora-Neto, N. Thakur, A. Elrefaey, J. Newman, Sneha Vishwanath, A. Nadesalingam, A. Chan, Peter Smith, J. Castillo-Olivares, H. Baxendale, B. Charleston, J. Heeney, D. Bailey, Nigel James Temperton

Communications Biology, 2021

Reduced neutralization of SARS-CoV-2 B.1.617 by vaccine and convalescent serum

Chang Liu, H. Ginn, W. Dejnirattisai, P. Supasa, Beibei Wang, A. Tuekprakhon, Rungtiwa Nutalai, D. Zhou, A. Mentzer, Yuguang Zhao, Helen M. E. Duyvesteyn, C. López-Camacho, J. Slon-Campos, T. Walter, D. Skelly, Síle A. Johnson, T. Ritter, Chris Mason, S. A. C. Clemens, F. Naveca, V. Nascimento, F. Nascimento, C. Costa, P. Resende, A. Pauvolid-Corrêa, M. Siqueira, C. Dold, Nigel James Temperton, T. Dong, A. Pollard, J. Knight, D. Crook, T. Lambe, E. Clutterbuck, S. Bibi, A. Flaxman, M. Bittaye, Sandra Belij-Rammerstorfer, S. Gilbert, Tariq Malik, M. Carroll, P. Klenerman, E. Barnes, S. Dunachie, V. Baillie, Natali Serafin, Z. Ditse, K. Silva, N. Paterson, Mark A. Williams, David Hall, S. Madhi, M. Nunes, P. Goulder, E. Fry, J. Mongkolsapaya, Jingshan Ren, D. Stuart, G. Screaton

Cell, 2021

The antibody response to SARS-CoV-2 Beta underscores the antigenic distance to other variants

Chang Liu, D. Zhou, Rungtiwa Nutalai, Helen M. E. Duyvesteyn, A. Tuekprakhon, H. Ginn, W. Dejnirattisai, P. Supasa, A. Mentzer, Beibei Wang, J. Case, Yuguang Zhao, D. Skelly, Rita E. Chen, Síle A. Johnson, T. Ritter, Chris Mason, Tariq Malik, Nigel James Temperton, N. Paterson, Mark A. Williams, David Hall, D. Clare, A. Howe, P. Goulder, E. Fry, M. Diamond, J. Mongkolsapaya, Jingshan Ren, D. Stuart, G. Screaton

Cell Host and Microbe, 2021

The antigenic anatomy of SARS-CoV-2 receptor binding domain

W. Dejnirattisai, D. Zhou, H. Ginn, Helen M. E. Duyvesteyn, P. Supasa, J. Case, Yuguang Zhao, T. Walter, A. Mentzer, Chang Liu, Beibei Wang, G. Paesen, J. Slon-Campos, C. López-Camacho, Natasha M. Kafai, Adam Leitman Bailey, Rita E. Chen, B. Ying, C. Thompson, Jai S Bolton, Alex J. Fyfe, Sunetra Gupta, T. Tan, Javier Gilbert-Jaramillo, W. James, M. Knight, M. Carroll, D. Skelly, C. Dold, Yanchun Peng, R. Levin, T. Dong, A. Pollard, J. Knight, P. Klenerman, Nigel James Temperton, D. Hall, Mark A. Williams, N. Paterson, F. Bertram, C. A. Siebert, D. Clare, A. Howe, J. Radecke, Yun-feng Song, A. Townsend, Kuan-Ying A. Huang, E. Fry, J. Mongkolsapaya, M. Diamond, Jingshan Ren, D. Stuart, G. Screaton

Cell, 2021

The role of pseudotype neutralization assays in understanding SARS CoV-2

D. Cantoni, M. Mayora-Neto, Nigel J. Temperton

Oxford open immunology, 2021

Characterisation of SARS-CoV-2 Lentiviral Pseudotypes and Correlation between Pseudotype-Based Neutralisation Assays and Live Virus-Based Micro Neutralisation Assays

I. Hyseni, E. Molesti, Linda Benincasa, Pietro Piu, Elisa Casa, Nigel James Temperton, A. Manenti, E. Montomoli

Viruses, 2020

Combined Point-of-Care Nucleic Acid and Antibody Testing for SARS-CoV-2 following Emergence of D614G Spike Variant

P. Mlcochova, D. Collier, A. Ritchie, S. Assennato, M. Hosmillo, N. Goel, B. Meng, Krishna Chatterjee, V. Mendoza, Nigel James Temperton, L. Kiss, L. James, K. Ciazynska, X. Xiong, J. Briggs, J. Nathan, F. Mescia, L. Bergamaschi, Hongyi Zhang, P. Barmpounakis, N. Demeris, R. Skells, P. Lyons, J. Bradley, Steven F. Baker, J. Allain, Kenneth G. C. Smith, R. Bousfield, M. Wilson, Dominic Sparkes, Glenn Amoroso, E. Gkrania-Klotsas, Susie Hardwick, A. Boyle, I. Goodfellow, Ravindra K. Gupta, Stephen Baker, G. Dougan, Ravindra K. Gupta, P. Lehner, P. Lyons, N. Matheson, M. Toshner, M. Weekes, Nick M Brown, M. Curran, Surendra Palmar, D. Enoch, D. Chapman, A. Shaw, Sherly Jose, A. Bermperi, Julie Ann Zerrudo, Evgenia Kourampa, L. Watson, Jieniean Worsley, C. Saunders, Ranalie de Jesus, Jason Domingo, C. Pasquale, B. Vergese, P. Vargas, Marivic Fabiculana, M. Perales, Lee Mynott, Elizabeth Blake, A. Bates, A. Vallier, Alexandria Williams, David Phillips, E. Chiu, A. Overhill, N. Ramenatte, J. Sipple, Steven G. Frost, H. Knock, R. Hardy, Emily Foster, Fiona Davidson, V. Rundell, P. Bundi, Richmond Abeseabe, S. Clark, I. Vicente, A. Elmer, C. Ribeiro, Jenny Kourampa, J. Kennet, J. Rowlands, A. Meadows, Criona O’Brien, Rebecca J Rastall, Cherry Crucusio, S. Hewitt, Jane Price, J. Calder, L. Canna, Ashlea Bucke, Hugo Tordesillas, Julie Harris, Valentina Ruffolo, B. Graves, H. Butcher, D. Caputo, Emma Le Gresley, B. Dunmore, Jennifer M. Martin, E. Legchenko, C. Treacy, Christopher Huang, Jennifer Wood, R. Sutcliffe, J. Hodgson, J. Shih, S. Graf, Z. Tong, F. Mescia, T. Tilly, Ciara O’Donnell, K. Hunter, L. Pointon, Nicole Pond, Marta Wylot, E. Jones, Stuart Fawke, Ben Bullman, L. Turner, Isobel Jarvis, Ommar Omarjee, Aloka De Sa, J. Marsden, Ariana Betancourt, M. Perera, M. Epping, N. Richoz, G. Bower, Rahul Sharma, F. Nice, O. Huhn, Natalia Savoinykh Yarkoni, N. Romashova, Dan Lewis, Andrew Hinch, C. Cossetti, Mateusz Strezlecki, R. Grenfell, H. Stark, N. Walker, K. Stirrups, Nigel R. Ovington, Eleanor Dewhust, Emily Li, S. Papadia, N. Kingston, A. Lever, E. Torok, William Hamilton, G. Hall, A. Jahun, Y. Chaudhry, M. Pinckert, I. Georgana, A. Yakovleva, L. Caller, S. Caddy, T. Feltwell, F. Khokhar, L. Meredith, Charlotte Holdcroft, S. Parmar

Cell Reports Medicine, 2020

Development of immunohistochemistry and in situ hybridisation for the detection of SARS-CoV and SARS-CoV-2 in formalin-fixed paraffin-embedded specimens

F. Lean, M. Lamers, Samuel P. Smith, R. Shipley, D. Schipper, Nigel James Temperton, B. Haagmans, A. Banyard, K. Bewley, M. Carroll, S. Brookes, I. Brown, A. Núñez

Scientific Reports, 2020

Evaluation of a Pseudotyped Virus Neutralisation Test for the Measurement of Equine Influenza Virus-Neutralising Antibody Responses Induced by Vaccination and Infection

R. Kinsley, S. Pronost, M. De Bock, Nigel James Temperton, J. Daly, R. Paillot, S. Scott

Vaccines, 2020

Protection From Influenza by Intramuscular Gene Vector Delivery of a Broadly Neutralizing Nanobody Does Not Depend on Antibody Dependent Cellular Cytotoxicity

J. M. D. Del Rosario, Matthew M. Smith, K. Zaki, P. Risley, Nigel James Temperton, O. Engelhardt, M. Collins, Y. Takeuchi, S. Hufton

Frontiers in Immunology, 2020

Entry of the bat influenza H17N10 virus into mammalian cells is enabled by the MHC class II HLA-DR receptor

E. Giotis, G. Carnell, E. Young, Saleena Ghanny, P. Soteropoulos, Lin‐Fa Wang, W. Barclay, M. Skinner, N. Temperton

Nature Microbiology, 2019

Pseudotype Neutralization Assays: From Laboratory Bench to Data Analysis

F. Ferrara, N. Temperton

Methods and protocols, 2018

An Optimized Method for the Production Using PEI, Titration and Neutralizationof SARS-CoV Spike Luciferase Pseudotypes.

G. Carnell, K. Grehan, F. Ferrara, E. Molesti, N. Temperton

Bio-protocol, 2017

Chimeric influenza haemagglutinins: Generation and use in pseudotype neutralization assays

F. Ferrara, N. Temperton

MethodsX, 2016

An optimised method for the production of MERS-CoV spike expressing viral pseudotypes

K. Grehan, F. Ferrara, N. Temperton

MethodsX, 2015

Pseudotype-Based Neutralization Assays for Influenza: A Systematic Analysis

G. Carnell, F. Ferrara, K. Grehan, C. Thompson, N. Temperton

Front. Immunol., 2015

The use of pseudotypes to study viruses, virus sero-epidemiology and vaccination☆

E. Bentley, S. Mather, N. Temperton

Vaccine, 2015

Lyophilisation of influenza, rabies and Marburg lentiviral pseudotype viruses for the development and distribution of a neutralisation -assay-based diagnostic kit.

S. Mather, E. Wright, S. Scott, N. Temperton

Journal of virological methods, 2014

Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies

E. Molesti, E. Wright, C. Terregino, R. Rahman, G. Cattoli, N. Temperton

Journal of immunology research, 2014

Comparative Serological Assays for the Study of H5 and H7 Avian Influenza Viruses

E. Molesti, A. Milani, C. Terregino, G. Cattoli, N. Temperton

Influenza research and treatment, 2013

Development of LPAI and HPAI H7 avian influenza pseudotypesfor serological applications utilising a combination of protease cotransfectionand site-directed mutagenesis

E. Molesti, G. Cattoli, F. Ferrara, E. Böttcher-Friebertshäuser, Calogero Terragino, N. Temperton

2013

The human Transmembrane Protease Serine 2 is necessary for the production of Group 2 influenza A virus pseudotypes

F. Ferrara, E. Molesti, E. Böttcher-Friebertshäuser, G. Cattoli, D. Corti, S. Scott, N. Temperton

Journal of molecular and genetic medicine : an international journal of biomedical research, 2013

The production and development of H7 Influenza virus pseudotypes for the study of humoral responses against avian viruses

E. Molesti, G. Cattoli, F. Ferrara, E. Böttcher-Friebertshäuser, C. Terregino, N. Temperton

Journal of molecular and genetic medicine : an international journal of biomedical research, 2013

The Use of Retroviral Pseudotypes for the Measurement of Antibody Responses to SARS Coronavirus

N. Temperton

Molecular Biology of the SARS-Coronavirus, 2009

A sensitive retroviral pseudotype assay for influenza H5N1‐neutralizing antibodies

N. Temperton, K. Hoschler, D. Major, C. Nicolson, R. Manvell, V. Hien, D. Ha, M. D. de Jong, M. Zambon, Y. Takeuchi, R. Weiss

Influenza and other respiratory viruses, 2007

Longitudinally Profiling Neutralizing Antibody Response to SARS Coronavirus with Pseudotypes

N. Temperton, P. Chan, G. Simmons, M. Zambon, R. Tedder, Y. Takeuchi, R. Weiss

Emerging infectious diseases, 2005

Enhancement of humoral immune responses to a human cytomegalovirus DNA vaccine: Adjuvant effects of aluminum phosphate and CpG oligodeoxynucleotides

N. Temperton, D. Quenelle, K. Lawson, J. Zuckerman, E. Kern, P. Griffiths, V. Emery

Journal of medical virology, 2003

DNA vaccines against cytomegalovirus: current progress.

N. Temperton

International journal of antimicrobial agents, 2002

Overexpression of superoxide dismutase in Trypanosoma cruzi results in increased sensitivity to the trypanocidal agents gentian violet and benznidazole.

N. Temperton, S. Wilkinson, D. Meyer, J. Kelly

Molecular and biochemical parasitology, 1998

Cloning of an Fe-superoxide dismutase gene homologue from Trypanosoma cruzi.

N. Temperton, S. Wilkinson, J. Kelly

Molecular and biochemical parasitology, 1996