Journal article
D. Cantoni, M. Mayora-Neto, Nigel J. Temperton
Oxford open immunology, 2021
Oxford open immunology, 2021
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APA
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Cantoni, D., Mayora-Neto, M., & Temperton, N. J. (2021). The role of pseudotype neutralization assays in understanding SARS CoV-2. Oxford Open Immunology.
Chicago/Turabian
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Cantoni, D., M. Mayora-Neto, and Nigel J. Temperton. “The Role of Pseudotype Neutralization Assays in Understanding SARS CoV-2.” Oxford open immunology (2021).
MLA
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Cantoni, D., et al. “The Role of Pseudotype Neutralization Assays in Understanding SARS CoV-2.” Oxford Open Immunology, 2021.
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@article{d2021a,
title = {The role of pseudotype neutralization assays in understanding SARS CoV-2},
year = {2021},
journal = {Oxford open immunology},
author = {Cantoni, D. and Mayora-Neto, M. and Temperton, Nigel J.}
}
Abstract
Neutralisation assays are crucial tools to quantify the presence of functional neutralising antibodies in serum samples. Since the SARS-CoV-2 virus (the causative agent of COVID-19) is designated as a category 3 biosafety level pathogen, pseudotyped viruses bearing the SARS-CoV-2 spike protein permit extensive and widespread serum/plasma screening in a BSL 2 laboratory. These assays can be used to assess viral tropism, vaccine immunogenicity, efficacy of antiviral compounds (incl. therapeutic mAbs) and serosurveillance studies. In this article, we highlight approaches to SARS-CoV-2 viral pseudotyping, its practicality, and utility in increasing our understanding of neutralising antibodies against SARS-CoV-2.