Journal article
N. Temperton, D. Quenelle, K. Lawson, J. Zuckerman, E. Kern, P. Griffiths, V. Emery
Journal of medical virology, 2003
Journal of medical virology, 2003
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APA
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Temperton, N., Quenelle, D., Lawson, K., Zuckerman, J., Kern, E., Griffiths, P., & Emery, V. (2003). Enhancement of humoral immune responses to a human cytomegalovirus DNA vaccine: Adjuvant effects of aluminum phosphate and CpG oligodeoxynucleotides. Journal of Medical Virology.
Chicago/Turabian
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Temperton, N., D. Quenelle, K. Lawson, J. Zuckerman, E. Kern, P. Griffiths, and V. Emery. “Enhancement of Humoral Immune Responses to a Human Cytomegalovirus DNA Vaccine: Adjuvant Effects of Aluminum Phosphate and CpG Oligodeoxynucleotides.” Journal of medical virology (2003).
MLA
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Temperton, N., et al. “Enhancement of Humoral Immune Responses to a Human Cytomegalovirus DNA Vaccine: Adjuvant Effects of Aluminum Phosphate and CpG Oligodeoxynucleotides.” Journal of Medical Virology, 2003.
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@article{n2003a,
title = {Enhancement of humoral immune responses to a human cytomegalovirus DNA vaccine: Adjuvant effects of aluminum phosphate and CpG oligodeoxynucleotides},
year = {2003},
journal = {Journal of medical virology},
author = {Temperton, N. and Quenelle, D. and Lawson, K. and Zuckerman, J. and Kern, E. and Griffiths, P. and Emery, V.}
}
Abstract
A human cytomegalovirus (HCMV) glycoprotein B (gpUL55) DNA vaccine has been evaluated in BALB/c mice. Intramuscular immunization of these mice with pRc/CMV2‐gB resulted in the generation of high levels of gpUL55‐specific antibody (geometric mean titer [GMT] 1:8900) and neutralizing antibody (GMT 1:74) after 2 booster doses given 5 and 10 weeks after primary inoculation. Emulsifying the construct with the aluminum phosphate gel adjuvant Adju‐Phos before immunization enhanced gpUL55‐specific antibody responses (GMT 1:17800, P = 0.04). Co‐immunization with CpG oligodeoxynucleotides was shown to enhance levels of neutralizing antibodies generated by immunization of mice with a pRc/CMV2‐gB/Adju‐Phos emulsion (P = 0.04). The results provide a rationale for evaluating combinations of other HCMV proteins for incorporation into a multi‐target DNA vaccine, and for the optimization of adjuvant usage, to elicit enhanced levels of neutralizing antibodies. J. Med. Virol. 70:86–90, 2003. © 2003 Wiley‐Liss, Inc.