Journal article
G. Carnell, K. Grehan, F. Ferrara, E. Molesti, N. Temperton
Bio-protocol, 2017
Bio-protocol, 2017
Semantic Scholar
DOI
PubMed
Cite
Cite
APA
Click to copy
Carnell, G., Grehan, K., Ferrara, F., Molesti, E., & Temperton, N. (2017). An Optimized Method for the Production Using PEI, Titration and Neutralizationof SARS-CoV Spike Luciferase Pseudotypes. Bio-Protocol.
Chicago/Turabian
Click to copy
Carnell, G., K. Grehan, F. Ferrara, E. Molesti, and N. Temperton. “An Optimized Method for the Production Using PEI, Titration and Neutralizationof SARS-CoV Spike Luciferase Pseudotypes.” Bio-protocol (2017).
MLA
Click to copy
Carnell, G., et al. “An Optimized Method for the Production Using PEI, Titration and Neutralizationof SARS-CoV Spike Luciferase Pseudotypes.” Bio-Protocol, 2017.
BibTeX Click to copy
@article{g2017a,
title = {An Optimized Method for the Production Using PEI, Titration and Neutralizationof SARS-CoV Spike Luciferase Pseudotypes.},
year = {2017},
journal = {Bio-protocol},
author = {Carnell, G. and Grehan, K. and Ferrara, F. and Molesti, E. and Temperton, N.}
}
Abstract
The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection reagent PEI. This protocol is optimized for transfection in 6-well plates; however it can be readily scaled to different production volumes according to application. This protocol has multiple benefits including the use of readily available reagents, consistent, high pseudotype virus production Relative Luminescence Units (RLU) titres and rapid generation of novel coronavirus pseudotypes for research into strain variation, tropism and immunogenicity/sero-prevalence.